Adipocytes - Stem Cells
Liposuction removes adipose tissue consisting of stromal vascular fraction (SVF), mature adipocytes and extracellular matrix (ECM). SVF is a rich source of pluripotent stem cells of adipose tissue ADSC (Adipose-Derived Stem Cells), with high regenerative potential. Studies show that regenerative properties are found in both isolated ADSCs and the original, mixed SVF cell population of the liposuction from which they are derived. This regenerative mixture contains many cell populations including mesenchymal stem cells (MSCs), endothelial progenitor cells (EPCs), immune cells, smooth muscle cells, pericytes, and other stromal components.
Both isolated ADSC and SVF mixtures increase the rate of healing and reduce pain and recovery time, differentiating into specific cell lines to repair the damage. The natural, concomitant release of cytokines and growth factors contributes to this, which we further enhance by combining PRP with the use of ADSC and SVF products. Depending on the application, the two sample types may be superior to each other. Amvyx offers us the option of preparing both types of fat stem cells at the point of care (point of care), within 4th generation, 100% closed systems, with safety, speed and repeatable performance, without the need for tissue processing or culture in a laboratory by a specialist staff.
LIPOPLEXTM: Combination of fat transfer and SVF stem cells
With the LIPOPLEXTM kit, subcutaneous fat is collected by suction through a special, thin, non-traumatic, multi-perforated cannula, which is transferred aseptically to the special dual separation container. After a few minutes of centrifugation, the unwanted fractions of the sample (water, blood, etc.) are removed through the dual access valves. The remaining material is centrifuged and at the same time homogenized and emulsified by filtration into a special container, ready for use. The LIPOPLEXTM kit offers the possibility of three (3) emulsification levels: (a) macroFAT, (b) microFAT and (c) nanoSVF.
Homogeneous macroFAT and microFAT maintain intact adipocytes and are suitable for lipotransfer applications that combine tissue regeneration. MacroFAT is used for volume in extended disease, injury or aging deficits with non-traumatic cannulas ± 2 mm in diameter, while microFAT is used for precision applications on eyelashes and lips with 0.7mm diameter non-traumatic cannulas or sharp 23G deep needles for deep wrinkles.
The special composition of LIPOPLEXTM nanoSVF is extremely low in adipocytes and rich in CD34 + ADSC stem cells, with proven efficacy in cases where we prioritize tissue regeneration, such as skin rejuvenation, scar healing, skin grafting and trauma treatment, lichen therapy and other cases of regeneration. The combination of nanoSVF with PRP provides an ultimate tissue engineering tool, where we take advantage of the amazing proliferative capacity of adipose stem cells to differentiate into the mesoderm, ectoderm and intradermal lines.
STEMPLEXTM: ADSC Isolation. Absolute power, minimum volume.
With the STEMPLEXTM kit, subcutaneous fat is collected by suction through a special, thin, non-traumatic, multi-perforated cannula, which is transferred aseptically to the special double separation container. After a few minutes of centrifugation, the unwanted fractions of the sample (water, blood, etc.) are removed through the dual access valves. The remaining material is incubated in the container with a special enzyme, under agitation, to break down the adipose tissue and release the bound ADSC from the SVF. It is then subjected to cycles of rinsing and centrifugation, and finally a small, homogeneous volume of ultra-high concentration of isolated, pluripotent ADSC stem cells is extracted.
The ADSCs obtained from the STEMPLEXTM kit are ideal for tissue healing with small volume injections. Regeneration is a complex process of inflammation, angiogenesis, new tissue formation and eventual remodeling. ADSCs are integrated into the local environment and contribute to wound and tissue healing. By secreting cell communication molecules and cytokines, ADSCs accelerate repair by differentiating the required neighboring cells. In addition to facilitating wound healing, ADSCs can also recruit and stimulate endogenous stem cells to participate in the process.
ADSC applications extend to the full range of tissue engineering including angiogenesis, peripheral nerve, tendon, cartilage, skeletal muscle and skin regeneration. Contact our representatives for further information on the combined use of ADSC with tissue scaffolding and surgical applications.
